Description

Illumina NovaSeq 6000 Genomic Sequencing System

Overview:

The Illumina NovaSeq 6000 is a flexible genomic sequencing platform designed for both small and large-scale projects. It supports a wide range of sequencing applications, making it suitable for diverse research needs.

Technical Specifications:

• Flow Cell Configurations: The system operates with dual flow cells (S1, S2, S4), offering varied throughput capacities.
• Read Lengths: Users can configure read lengths to 2×50 bp, 2×100 bp, or 2×150 bp to suit specific project requirements.
• Throughput: The S1 flow cell handles up to 1.6 billion reads, generating 500 Gb. The S2 flow cell processes up to 3.3 billion reads, producing 1 Tb. The S4 flow cell manages up to 10 billion reads, yielding 3 Tb per run.
• Accuracy: It delivers a high sequencing accuracy, with over 80% of reads achieving a Q30 quality score.
• Run Time: Depending on the flow cell and read length, run times range from 13 to 44 hours.

Functional Attributes:

• Scalable Throughput: The NovaSeq 6000 adjusts to varying project sizes, which ensures its adaptability across different research applications.
• Versatile Sample Handling: It accommodates a variety of samples, including DNA and RNA, enabling broad usage in genomic studies.
• Efficient Data Generation: Capable of producing between 500 Gb and 6 Tb per run, the system meets the demands of extensive sequencing projects.

Connectivity Options:

• Data Management Integration: The system integrates seamlessly with Illumina’s BaseSpace Sequence Hub, which facilitates cloud-based storage, analysis, and collaboration.
• Network Connectivity: Equipped with dual 1 Gb Ethernet ports and optional 10 Gb Ethernet, it allows for quick data transfer and remote monitoring.
• Export Formats: The system supports standard output formats like FASTQ, BAM, and VCF, ensuring compatibility with existing bioinformatics tools.

Use Cases:

Researchers use the NovaSeq 6000 for high-throughput sequencing tasks, such as whole-genome and exome sequencing. Its scalability and flexibility make it a preferred tool in both research and clinical environments. https://sapac.support.illumina.com/content/dam/illumina-support/documents/documentation/system_documentation/novaseq/1000000019358_17_novaseq-6000-system-guide.pdf